Mutation Counter
Introduction
This page is provided for convinience, but it is reccomended that you verify manually with your actual trace files to determine whether a mutation is not a Sanger sequencing artefact. My reccomendation is that you open the ABI files and check what each mutation listed below looks like.
Prepare sequences
Get ref.
then twice (for forward and reverse): seq, option for no change, add (primer) or remove (barcode)
results for alignement
Prep_out
Does this align it? The alignment is pairwise and need not be sophisticated. 2 consecutive mismatchs. insertion/deletion. Trim, find 5 conserved.
AB file to XML. what if one could show the base in question. it depends on the XML
Aligned Sequences
Wild type sequence
Mutant sequences